Example of Intracellular Cytokine Staining

    Tetramer staining of fresh or frozen CD8 + T cells, before and after in vitro expansion with EBV peptide

    Frozen CD8+ T cells are as functional as fresh CD8+ T cells by ELISpot

    IFN-gamma ELISpot of CD8+ T cells from a melanoma patient immunized with tumor peptide (Melan-A)

    Western blotting for HDJ-2 as an indicator of farnesylation status

    Dose response in K562 cells and patient samples