In general, assays and procedures are developed as needed for the execution of new clinical studies. Several procedures are currently performed on a routine basis, and multiple assays are under development for pending clinical projects.

Current Assays and Procedures

• Antigen-specific cytokine production (using ELISpot analysis) and CTL activity of freshly isolated or in vitro expanded T cells
• PHA-stimulated T cell proliferation
• Serum analysis of cytokines by ELISA or Cytokine Protein Array
• Flow cytometric analysis of hematopoietic and T cell subsets, including peptide/HLA tetramer staining of T cells
• Induced intracellular cytokines by flow cytometry
• RT-PCR for detection of tumor antigen gene expression
• Western blot analysis from tumor specimens or PBMC
• Antibody-dependent cellular cytotoxicity (ADCC) assay
• Immunohistochemical analysis of tumor biopsies (e.g. expression of HLA, TAP, tumor antigens, apoptotic cells)
• Real-time RT-PCR analysis of gene expression in tumor biopsies
• Expansion of clinical grade dendritic cell subsets
• RNA preparation for gene microarray analysis

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