Sample Processing

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Or for a downloadable spreadsheet of our price list click here.

 
Sample Processing & Storage Sample type
Isolating and freezing PBMCs (50 ml blood; Lymphoprep) 4-5 green top tubes */ 1 x 40-50 ml heparinzed syringe
Isolating and freezing PBMCs (100 ml blood; Lymphoprep) 2 x 50 ml heparinzed syringe
Isolating and freezing PBMCs from 1 x CPT CPT or 1-2 green top tubes *
Isolating and freezing PBMCs from 4 x CPT CPT (red/green or blue/black swirl)**
Isolating and freezing serum/plasma 1 red top / 1 green top *
CD3 + separation (StemSep or Easy Sep) PBMCs ***
CD8 + separation (MACs) PBMCs
Liquid nitrogen storage/month PBMCs, Cell lines
FACS-based Analysis
Surface staining (eg CD3, CD14, CD15, CD19, CD56) PBMCs or whole blood
Surface staining (eg CD3, CD4) PBMCs or whole blood
Tetramer staining (excludes tetramer cost) PBMCs
T2 Binding Assay Peptide
HLA-A2 Screening Whole blood, PBMCs
Intracellular staining (T-reg - FoxP3+CD25+CD4) PBMCs
Intracellular staining (IL-5 and IFN-g) Whole blood, PBMCs
Luminex Assay Cell Lysates
Molecular Immunology
RNA isolation and quantitation (from tissue biopsies) Tissue Biopsy
RNA isolation and quantiation (from PBMCs) PBMCs
cDNA synthesis (up to 4 samples) Isolated RNA
PCR/experiment
Standard Assays
ELISPOT (basic) PBMCs
ELISPOT (IFN-g) PBMCs
Western Blot (ABs/stimulation not included) PBMCs or Cell lines
ELISA (Abs not included) Serum/Plasma/Supernatants
Vaccine Preparation
Peptide Preparation (excluding peptide cost/cGMP fee) Peptide
Peptide Preparation (including QC testing) Peptide
Single Peptide vaccine (excluding peptide cost) Peptide & adjuvants
Miscellaneous Tests

Endosafe Endotoxin test (per cartridge) Test sample
LAL Gel Clot Endotoxin test (including standard curve) Test sample
Machine Costs

Coulter Counter - Differential Count (ONLY human blood) ONLY human blood
ELISPOT Machine - reserve machine via scheduler

* BD vacutainer

** CPT = Cell Preparation Tubes from BD

*** PBMCs = Peripheral Blood Mononuclear Cells

Note: We also offer other services not listed above, such as cell culture, semi-cloning, real-time PCR, and many more. Also, please note that additional charges may apply depending on the specific reagents used.

Please contact us for more information and to help us serve you better.